|
KMID : 0043320090320060831
|
|
Archives of Pharmacal Research
2009 Volume.32 No. 6 p.831 ~ p.840
|
|
|
Isolation and Tandem Mass Fragmentations of an Anti-inflammatory Compound from Aralia elata
|
|
Park You-Mie
Kim Yeong-Shik
Jeong Choon-Sik
Ha Young-Wan
Lee Ju-Hyeon
|
|
|
Abstract
|
|
|
|
One-step isolation of a saponin from Aralia elata was undertaken using high-speed countercurrent chromatography coupled with evaporative light scattering detection. A triterpenoid saponin, elatoside F, was purified with 96.8% purity using a two-phase-system comprising chloroform-methanol-water-isopropanol. The yield was 35.0 mg from 348.2 mg of the enriched saponin fraction. In vitro anti-inflammatory study demonstrated that elatoside F inhibited lipopolysaccharide-induced nitric oxide production, as well as nuclear factor κB activation, in a dose-dependent manner. Two types of mass ionization technique were compared on elatoside F to investigate characteristic fragmentation patterns. MALDI-TOF tandem mass spectrometric fragmentation patterns of sodiated ions provided structural information on glycosidic cleavages and on extensive cross-ring cleavages. Electrospray ionization multiple-stage tandem mass fragmentation of both sodiated and lithiated ions could provide information on glycosidic cleavages. All observed tandem mass fragmentation spectra provided valuable elatoside F structural information when unknown samples from crude extracts are under screening by mass spectrometry.
|
|
|
KEYWORD
|
|
|
Aralia elata, In vitro anti-inflammatory activity, High-speed counter-current chromatography, Tandem mass spectrometry, Triterpenoid saponin, Elatoside F
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|